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SfN 2017 Poster Presentation

Sunday Nov 12th 2017

1pm-5pm

AA5 224.02 – Integration of bilateral tactile stimuli in the somatosensory cortex of the awake mouse
*A. PALA, B. CHEN, C. J. WHITMIRE, G. B. STANLEY;
Biomed. Engin., Georgia Inst. of Technol., Atlanta, GA
AA6 224.03 – Pushing the perceptual boundary towards optimal performance in a detection task with changing stimulus statistics
*C. WAIBLINGER, P. Y. BORDEN, M. F. BOLUS, G. B. STANLEY;
Biomed. Engin., Georgia Inst. of Technol., Atlanta, GA
AA7 224.04 – A predictive framework to define the spatial and temporal scale of local cortical state in the awake animal from multi-electrode array recordings in mouse somatosensory cortex
*A. J. SEDERBERG, A. PALA, G. B. STANLEY;
Biomed. Engin., Georgia Inst. of Technol., Atlanta, GA

 

Monday Nov 13th 2017

8am-12pm

WW5 345.02 – Capping patch clamp pipettes for improved gigaseal yield
*W. STOY1, I. KOLB1, G. L. HOLST2, G. B. STANLEY1, C. FOREST2;
1Wallace H. Coulter Dept. of Biomed. Engin., 2George W. Woodruff Sch. of Mechanical Engin., Georgia Inst. of Technol., Atlanta, GA

 

Tuesday Nov 14th 2017

1pm-5pm

BB18 583.02 – Quantifying sensory encoding from thalamus to cortex in topographically aligned neuron pairs
*Y. LIEW1, C. J. WHITMIRE1, A. PALA1, W. A. STOY1, P. Y. BORDEN1, A. D. ORTIZ1, B. YANG2, C. R. FOREST1,2, G. B. STANLEY1;
1Wallace H Coulter Dept. of Biomed. Engin., 2George W Woodruff Sch. of Mechanical Engin., Georgia Inst. of Technol., Atlanta, GA
BB19 583.03 – Thalamic state modulation of somatosensory encoding in the thalamocortical circuit
*C. J. WHITMIRE, Y. LIEW, A. PALA, G. B. STANLEY;
Biomed. Engin., Georgia Inst. of Technol., Atlanta, GA
BB20 583.04 – Thalamic control of sensory evoked spatiotemporal cortical responses
*P. Y. BORDEN1, I. KOLB1, A. D. ORTIZ1, A. J. SEDERBERG1, C. WAIBLINGER1, W. STOY1, C. FOREST2,3, A. MORRISSETTE4, D. JAEGER4, G. B. STANLEY1;
1Georgia Inst. of Technol., Atlanta, GA; 2Georgia Tech., Atlanta, GA; 3Mechanical Engin., Georgia Inst. Of Technol., Atlanta, GA; 4Biol., Emory Univ., Atlanta, GA
BB21 583.05 – Closed loop optogenetic control of thalamocortical activity
*M. F. BOLUS1, A. A. WILLATS1, C. J. WHITMIRE1, C. J. ROZELL2, G. B. STANLEY1;
1Biomed. Engin., 2Sch. of Electrical & Computer Engin., Georgia Inst. of Technol., Atlanta, GA

 

Stanley Lab Welcomes ENGAGES Student Zaria Hardnett

The Stanley Lab is excited to welcome Zaria Hardnett, through Project ENGAGES (http://projectengages.gatech.edu/).  Zaria will be working with the Stanley Lab this year alongside mentor Michael Bolus, getting hands-on experience conducting biological/engineering research.

Zaria is a rising senior at Benjamin E. Mays High School, enrolled in the Science and Mathematics Academy there. She is interested in biotechnology more generally and has previously  interned at a patent law office.

Recently, at the Project ENGAGES Summer Celebration, Zaria’s poster presentation took home first prize which includes an Amazon gift card! She presented preliminary work doing neural data processing in front of a group of peers, community members and family. Congratulations Zaria!

New Publication – Genetically expressed voltage sensor ArcLight for imaging large scale cortical activity in the anesthetized and awake mouse

With the recent breakthrough in genetically expressed voltage indicators (GEVIs), there has been a tremendous demand to determine the capabilities of these sensors in vivo. Novel voltage sensitive fluorescent proteins allow for direct measurement of neuron membrane potential changes through changes in fluorescence. Here, we utilized ArcLight, a recently developed GEVI, and examined the functional characteristics in the widely used mouse somatosensory whisker pathway. We measured the resulting evoked fluorescence using a widefield microscope and a CCD camera at 200 Hz, which enabled voltage recordings over the entire cortical region with high temporal resolution. We found that ArcLight produced a fluorescent response in the S1 barrel cortex during sensory stimulation at single whisker resolution. During wide-field cortical imaging, we encountered substantial hemodynamic noise that required additional post hoc processing through noise subtraction techniques. Over a period of 28 days, we found clear and consistent ArcLight fluorescence responses to a simple sensory input. Finally, we demonstrated the use of ArcLight to resolve cortical S1 sensory responses in the awake mouse. Taken together, our results demonstrate the feasibility of ArcLight as a measurement tool for mesoscopic, chronic imaging. © 2017 Society of Photo-Optical Instrumentation Engineers (SPIE) [DOI: 10.1117/1.NPh.4.3.031212]

P. Y. Borden, A. D. Ortiz, C. Waiblinger, A. J. Sederberg, A. Morrissette, C. Forest, D. Jaeger, G. B. Stanley, Genetically Expressed Voltage Sensor ArcLight for Imaging Large Scale Cortical Activity in the Anesthetized and Awake Mouse, Neurophotonics 4(3), 031212, 2017. PDF

PhD Proposal Announcement: Peter Borden

Peter Borden 
BME PhD Thesis Proposal Presentation
Date and Time:Thursday, March 2nd, 10-11am 
Location: Emory Rollins Research Center 1052 
Committee:
Garrett Stanley (advisor)
Dieter Jaeger                                                 
Robert Liu                                                  
Bilal Haider
Biyu He (NYU) 

Title: The Impact of Thalamic State on Sensory Cortical Processing and Behavior

The thalamus is a central junction that processes both sensory afferent and motor efferent signals. Although many neurological disorders including Parkinson’s disease, Schizophrenia, and Central Pain are linked to thalamic dysfunction, basic information about thalamic processing is still unknown. Specifically, it is unclear how ongoing changes in membrane polarization (i.e. state) alter the transmission of information to and from cortical regions. Thalamic neurons have dynamic firing modes (i.e. tonic and burst) and receive tremendous amounts of neuromodulatory inputs that shape the encoding of sensory features. My project will develop novel techniques to measure entire cortical regions and use these tools determine the role of thalamic state on tactile processing and detectability of sensory inputs. Specifically, I utilize the novel genetically expressed voltage indicator ArcLight to measure voltage activity across cortical structures. I will record cortical ArcLight signals while simultaneously manipulating the ongoing thalamic activity using genetically expressed light sensitive protein channels (optogenetics). I will further combine these techniques to modulate thalamic state to control the evoked cortical response and behavioral performance of mice during a tactile detection task.  It is critical that we understand how thalamic state alters information transmission to develop better treatment options for these complex neurological disorders.

Stanley Lab Welcomes New Postdoc Caleb Wright

The Stanley lab is excited to welcome our new post-doctoral researcher,  Nathaniel (Caleb) Wright!

Nathaniel received his PhD in Physics from Washington University in St. Louis.  He worked in Ralf Wessel’s lab, where he performed multi-whole-cell and local field potential recordings of visual responses in cortex.  He and his collaborators combined these recordings with network simulations to study the dynamics and mechanisms of cortical coordination across multiple spatial scales during visual processing.