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Stanley Laboratory Awarded New $1.9M NIH-NINDS BRAIN Initiative Award!

The laboratory of Prof. Garrett Stanley in the Coulter Department of Biomedical Engineering at Georgia Tech and Emory University was awarded a 5 year, $1.9M grant through the “BRAIN Initiative: Targeted BRAIN Circuits Projects – TargetedBCP” program. The title of the project is “Thalamocortical state control of tactile sensing: Mechanisms, Models, and Behavior”.  The team proposes to utilize an array of electrophysiological tools to measure and manipulate the circuits on fast time scales, to determine the role the thalamus has in dynamically gating information flow to the rest of the brain during changes in states of arousal.

Aurélie Pala awarded Swiss National Science Foundation Advanced Postdoc Mobility Fellowship

Aurélie Pala, a postdoc in the Stanley Lab was awarded a Swiss National Science Foundation (SNSF) Advanced Postdoc Mobility Fellowship for her project entitled “The Neuronal Correlates and Computations underlying Bilateral Tactile Perception”. The fellowship will provide funding for 1.5 years and will enable her to pursue her research on the neuronal basis of cerebral inter-hemispheric interactions contributing to the sense of touch.

Details on the award can be found on the SNSF website.

SfN 2017 Poster Presentation & Talks

Thursday Nov 9th 2017

Barrels XXX Satellite Conference

Glass Pavilion at the Homewood Campus of
Johns Hopkins University, Baltimore, Maryland

12:20pm

Invited talk: Pushing the perceptual boundary towards optimal performance in a detection task with changing stimulus statistics
C. WAIBLINGER, P. Y. BORDEN, M. F. BOLUS, G. B. STANLEY;

Sunday Nov 12th 2017

1pm-5pm

AA5 224.02 – Integration of bilateral tactile stimuli in the somatosensory cortex of the awake mouse
*A. PALA, B. CHEN, C. J. WHITMIRE, G. B. STANLEY;
Biomed. Engin., Georgia Inst. of Technol., Atlanta, GA
AA6 224.03 – Pushing the perceptual boundary towards optimal performance in a detection task with changing stimulus statistics
*C. WAIBLINGER, P. Y. BORDEN, M. F. BOLUS, G. B. STANLEY;
Biomed. Engin., Georgia Inst. of Technol., Atlanta, GA
AA7 224.04 – A predictive framework to define the spatial and temporal scale of local cortical state in the awake animal from multi-electrode array recordings in mouse somatosensory cortex
*A. J. SEDERBERG, A. PALA, G. B. STANLEY;
Biomed. Engin., Georgia Inst. of Technol., Atlanta, GA

 

Monday Nov 13th 2017

8am-12pm

WW5 345.02 – Capping patch clamp pipettes for improved gigaseal yield
*W. STOY1, I. KOLB1, G. L. HOLST2, G. B. STANLEY1, C. FOREST2;
1Wallace H. Coulter Dept. of Biomed. Engin., 2George W. Woodruff Sch. of Mechanical Engin., Georgia Inst. of Technol., Atlanta, GA

 

Tuesday Nov 14th 2017

1pm-5pm

BB18 583.02 – Quantifying sensory encoding from thalamus to cortex in topographically aligned neuron pairs
*Y. LIEW1, C. J. WHITMIRE1, A. PALA1, W. A. STOY1, P. Y. BORDEN1, A. D. ORTIZ1, B. YANG2, C. R. FOREST1,2, G. B. STANLEY1;
1Wallace H Coulter Dept. of Biomed. Engin., 2George W Woodruff Sch. of Mechanical Engin., Georgia Inst. of Technol., Atlanta, GA
BB19 583.03 – Thalamic state modulation of somatosensory encoding in the thalamocortical circuit
*C. J. WHITMIRE, Y. LIEW, A. PALA, G. B. STANLEY;
Biomed. Engin., Georgia Inst. of Technol., Atlanta, GA
BB20 583.04 – Thalamic control of sensory evoked spatiotemporal cortical responses
*P. Y. BORDEN1, I. KOLB1, A. D. ORTIZ1, A. J. SEDERBERG1, C. WAIBLINGER1, W. STOY1, C. FOREST2,3, A. MORRISSETTE4, D. JAEGER4, G. B. STANLEY1;
1Georgia Inst. of Technol., Atlanta, GA; 2Georgia Tech., Atlanta, GA; 3Mechanical Engin., Georgia Inst. Of Technol., Atlanta, GA; 4Biol., Emory Univ., Atlanta, GA
BB21 583.05 – Closed loop optogenetic control of thalamocortical activity
*M. F. BOLUS1, A. A. WILLATS1, C. J. WHITMIRE1, C. J. ROZELL2, G. B. STANLEY1;
1Biomed. Engin., 2Sch. of Electrical & Computer Engin., Georgia Inst. of Technol., Atlanta, GA

 

Stanley Lab Welcomes ENGAGES Student Zaria Hardnett

The Stanley Lab is excited to welcome Zaria Hardnett, through Project ENGAGES (http://projectengages.gatech.edu/).  Zaria will be working with the Stanley Lab this year alongside mentor Michael Bolus, getting hands-on experience conducting biological/engineering research.

Zaria is a rising senior at Benjamin E. Mays High School, enrolled in the Science and Mathematics Academy there. She is interested in biotechnology more generally and has previously  interned at a patent law office.

Recently, at the Project ENGAGES Summer Celebration, Zaria’s poster presentation took home first prize which includes an Amazon gift card! She presented preliminary work doing neural data processing in front of a group of peers, community members and family. Congratulations Zaria!

New Publication – Genetically expressed voltage sensor ArcLight for imaging large scale cortical activity in the anesthetized and awake mouse

With the recent breakthrough in genetically expressed voltage indicators (GEVIs), there has been a tremendous demand to determine the capabilities of these sensors in vivo. Novel voltage sensitive fluorescent proteins allow for direct measurement of neuron membrane potential changes through changes in fluorescence. Here, we utilized ArcLight, a recently developed GEVI, and examined the functional characteristics in the widely used mouse somatosensory whisker pathway. We measured the resulting evoked fluorescence using a widefield microscope and a CCD camera at 200 Hz, which enabled voltage recordings over the entire cortical region with high temporal resolution. We found that ArcLight produced a fluorescent response in the S1 barrel cortex during sensory stimulation at single whisker resolution. During wide-field cortical imaging, we encountered substantial hemodynamic noise that required additional post hoc processing through noise subtraction techniques. Over a period of 28 days, we found clear and consistent ArcLight fluorescence responses to a simple sensory input. Finally, we demonstrated the use of ArcLight to resolve cortical S1 sensory responses in the awake mouse. Taken together, our results demonstrate the feasibility of ArcLight as a measurement tool for mesoscopic, chronic imaging. © 2017 Society of Photo-Optical Instrumentation Engineers (SPIE) [DOI: 10.1117/1.NPh.4.3.031212]

P. Y. Borden, A. D. Ortiz, C. Waiblinger, A. J. Sederberg, A. Morrissette, C. Forest, D. Jaeger, G. B. Stanley, Genetically Expressed Voltage Sensor ArcLight for Imaging Large Scale Cortical Activity in the Anesthetized and Awake Mouse, Neurophotonics 4(3), 031212, 2017. PDF