The Stanley Lab is excited to welcome Zaria Hardnett, through Project ENGAGES (http://projectengages.gatech.edu/). Zaria will be working with the Stanley Lab this year alongside mentor Michael Bolus, getting hands-on experience conducting biological/engineering research.
Zaria is a rising senior at Benjamin E. Mays High School, enrolled in the Science and Mathematics Academy there. She is interested in biotechnology more generally and has previously interned at a patent law office.
Recently, at the Project ENGAGES Summer Celebration, Zaria’s poster presentation took home first prize which includes an Amazon gift card! She presented preliminary work doing neural data processing in front of a group of peers, community members and family. Congratulations Zaria!
With the recent breakthrough in genetically expressed voltage indicators (GEVIs), there has been a tremendous demand to determine the capabilities of these sensors in vivo. Novel voltage sensitive fluorescent proteins allow for direct measurement of neuron membrane potential changes through changes in fluorescence. Here, we utilized ArcLight, a recently developed GEVI, and examined the functional characteristics in the widely used mouse somatosensory whisker pathway. We measured the resulting evoked fluorescence using a widefield microscope and a CCD camera at 200 Hz, which enabled voltage recordings over the entire cortical region with high temporal resolution. We found that ArcLight produced a fluorescent response in the S1 barrel cortex during sensory stimulation at single whisker resolution. During wide-field cortical imaging, we encountered substantial hemodynamic noise that required additional post hoc processing through noise subtraction techniques. Over a period of 28 days, we found clear and consistent ArcLight fluorescence responses to a simple sensory input. Finally, we demonstrated the use of ArcLight to resolve cortical S1 sensory responses in the awake mouse. Taken together, our results demonstrate the feasibility of ArcLight as a measurement tool for mesoscopic, chronic imaging. © 2017 Society of Photo-Optical Instrumentation Engineers (SPIE) [DOI: 10.1117/1.NPh.4.3.031212]
P. Y. Borden, A. D. Ortiz, C. Waiblinger, A. J. Sederberg, A. Morrissette, C. Forest, D. Jaeger, G. B. Stanley, Genetically Expressed Voltage Sensor ArcLight for Imaging Large Scale Cortical Activity in the Anesthetized and Awake Mouse, Neurophotonics 4(3), 031212, 2017. PDF
The Stanley lab is excited to welcome our new post-doctoral researcher, Nathaniel (Caleb) Wright!
Nathaniel received his PhD in Physics from Washington University in St. Louis. He worked in Ralf Wessel’s lab, where he performed multi-whole-cell and local field potential recordings of visual responses in cortex. He and his collaborators combined these recordings with network simulations to study the dynamics and mechanisms of cortical coordination across multiple spatial scales during visual processing.
The Stanley lab is happy to welcome visiting graduate student Linlin (Mia ) Lu from Peking University as part of the GT /PKU partnership.
Originally from China, Linlin Lu (Mia ), is a third year postgraduate student in the PKU-GT-Emory joint BME program. She received her bachelor’s degree of engineering in the department of Biomedical Engineering in Peking University in 2014 and continued her graduate studies in the laboratory of Prof. Duan Xiaojie at PKU. In the Duan lab, she focuses on developing flexible, soft and MRI compatible probes for chronic neural interface. As part of the Stanley lab she hopes to test the chronic recording capability and MRI compatibility of the probe in the whisker pathway, and try to combine the probe with other techniques, such as optogenetics and two-photon imaging, to develop better tools for use in neural science.