Poster & Workshop at COSYNE 2018

Friday March 2nd 2018

Adam Willats will present his work on state-aware control at
Poster II-38 State-aware control of neural activity: design & analysis. Adam Willats, Michael Bolus, Clarissa Whitmire, Garrett Stanley, Christopher Rozell

Tuesday March 6th 2018

Garrett Stanley and Christopher Rozell will host a workshop on closed-loop control of neural systems and circuits for scientific discovery. Student travel grants available thanks to IEEE Brain.

Stanley Laboratory Awarded New $1.9M NIH-NINDS BRAIN Initiative Award!

The laboratory of Prof. Garrett Stanley in the Coulter Department of Biomedical Engineering at Georgia Tech and Emory University was awarded a 5 year, $1.9M grant through the “BRAIN Initiative: Targeted BRAIN Circuits Projects – TargetedBCP” program. The title of the project is “Thalamocortical state control of tactile sensing: Mechanisms, Models, and Behavior”.  The team proposes to utilize an array of electrophysiological tools to measure and manipulate the circuits on fast time scales, to determine the role the thalamus has in dynamically gating information flow to the rest of the brain during changes in states of arousal.

More details can be found here

Aurélie Pala awarded Swiss National Science Foundation Advanced Postdoc Mobility Fellowship

Aurélie Pala, a postdoc in the Stanley Lab was awarded a Swiss National Science Foundation (SNSF) Advanced Postdoc Mobility Fellowship for her project entitled “The Neuronal Correlates and Computations underlying Bilateral Tactile Perception”. The fellowship will provide funding for 1.5 years and will enable her to pursue her research on the neuronal basis of cerebral inter-hemispheric interactions contributing to the sense of touch.

Details on the award can be found on the SNSF website.

SfN 2017 Poster Presentation & Talks

Thursday Nov 9th 2017

Barrels XXX Satellite Conference

Glass Pavilion at the Homewood Campus of
Johns Hopkins University, Baltimore, Maryland

12:20pm

Invited talk: Pushing the perceptual boundary towards optimal performance in a detection task with changing stimulus statistics
C. WAIBLINGER, P. Y. BORDEN, M. F. BOLUS, G. B. STANLEY;

Sunday Nov 12th 2017

1pm-5pm

AA5 224.02 – Integration of bilateral tactile stimuli in the somatosensory cortex of the awake mouse
*A. PALA, B. CHEN, C. J. WHITMIRE, G. B. STANLEY;
Biomed. Engin., Georgia Inst. of Technol., Atlanta, GA
AA6 224.03 – Pushing the perceptual boundary towards optimal performance in a detection task with changing stimulus statistics
*C. WAIBLINGER, P. Y. BORDEN, M. F. BOLUS, G. B. STANLEY;
Biomed. Engin., Georgia Inst. of Technol., Atlanta, GA
AA7 224.04 – A predictive framework to define the spatial and temporal scale of local cortical state in the awake animal from multi-electrode array recordings in mouse somatosensory cortex
*A. J. SEDERBERG, A. PALA, G. B. STANLEY;
Biomed. Engin., Georgia Inst. of Technol., Atlanta, GA

 

Monday Nov 13th 2017

8am-12pm

WW5 345.02 – Capping patch clamp pipettes for improved gigaseal yield
*W. STOY1, I. KOLB1, G. L. HOLST2, G. B. STANLEY1, C. FOREST2;
1Wallace H. Coulter Dept. of Biomed. Engin., 2George W. Woodruff Sch. of Mechanical Engin., Georgia Inst. of Technol., Atlanta, GA

 

Tuesday Nov 14th 2017

1pm-5pm

BB18 583.02 – Quantifying sensory encoding from thalamus to cortex in topographically aligned neuron pairs
*Y. LIEW1, C. J. WHITMIRE1, A. PALA1, W. A. STOY1, P. Y. BORDEN1, A. D. ORTIZ1, B. YANG2, C. R. FOREST1,2, G. B. STANLEY1;
1Wallace H Coulter Dept. of Biomed. Engin., 2George W Woodruff Sch. of Mechanical Engin., Georgia Inst. of Technol., Atlanta, GA
BB19 583.03 – Thalamic state modulation of somatosensory encoding in the thalamocortical circuit
*C. J. WHITMIRE, Y. LIEW, A. PALA, G. B. STANLEY;
Biomed. Engin., Georgia Inst. of Technol., Atlanta, GA
BB20 583.04 – Thalamic control of sensory evoked spatiotemporal cortical responses
*P. Y. BORDEN1, I. KOLB1, A. D. ORTIZ1, A. J. SEDERBERG1, C. WAIBLINGER1, W. STOY1, C. FOREST2,3, A. MORRISSETTE4, D. JAEGER4, G. B. STANLEY1;
1Georgia Inst. of Technol., Atlanta, GA; 2Georgia Tech., Atlanta, GA; 3Mechanical Engin., Georgia Inst. Of Technol., Atlanta, GA; 4Biol., Emory Univ., Atlanta, GA
BB21 583.05 – Closed loop optogenetic control of thalamocortical activity
*M. F. BOLUS1, A. A. WILLATS1, C. J. WHITMIRE1, C. J. ROZELL2, G. B. STANLEY1;
1Biomed. Engin., 2Sch. of Electrical & Computer Engin., Georgia Inst. of Technol., Atlanta, GA

 

New Publication – Robotic navigation to subcortical neural tissue for intracellular electrophysiology in vivo

Abstract: In vivo studies of neurophysiology using the whole cell patch-clamp technique enable exquisite access to both intracellular dynamics and cytosol of cells in the living brain but are underrepresented in deep subcortical nuclei because of fouling of the sensitive electrode tip. We have developed an autonomous method to navigate electrodes around obstacles such as blood vessels after identifying them as a source of contamination during regional pipette localization (RPL) in vivo. In mice, robotic navigation prevented fouling of the electrode tip, increasing RPL success probability 3 mm below the pial surface to 82% (n = 72/88) over traditional, linear localization (25%, n = 24/95), and resulted in high-quality thalamic whole cell recordings with average access resistance (32.0 MΩ) and resting membrane potential (−62.9 mV) similar to cortical recordings in isoflurane-anesthetized mice. Whole cell yield improved from 1% (n = 1/95) to 10% (n = 9/88) when robotic navigation was used during RPL. This method opens the door to whole cell studies in deep subcortical nuclei, including multimodal cell typing and studies of long-range circuits.

New & noteworthy: This work represents an automated method for accessing subcortical neural tissue for intracellular electrophysiology in vivo. We have implemented a novel algorithm to detect obstructions during regional pipette localization and move around them while minimizing lateral displacement within brain tissue. This approach leverages computer control of pressure, manipulator position, and impedance measurements to create a closed-loop platform for pipette navigation in vivo. This technique enables whole cell patching studies to be performed throughout the living brain.

W. A. Stoy, I. Kolb, G. L. Holst, Y. Liew, A. Pala, B. Yang, E. S. Boyden, G. B. Stanley, C. R. Forest
Journal of Neurophysiology Published 1 August 2017 Vol. 118 no. 2, 1141-1150 DOI: 10.1152/jn.00117.2017 PDF